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Then a biotinylated detection antibody specific for CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of MSX2 in the sample by plotting standard curve
Calculate the concentration of DAP6 in the sample by plotting standard curve
And then biotinylated Human GPR37 Antibody is added and binds to GPR37 in the sample
And then biotinylated Human MSRA Antibody is added and binds to MSRA in the sample
Human WNT10B (Protein Wnt-10b) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human WNT10B (Protein Wnt 10b) ELISA Kit measures WNT10B in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti WNT10B antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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